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Tag-lite plasmid featuring a HALO-Tag sequence, a T8 peptide sequence, and a Zeocin resistance gene.Cloning and expression of the GPCR of interest as a SNAP-Tag or CLIP-Tag fusion protein enables its subsequent labeling with Terbium.
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the road to the development of many non-radioactive, no-wash binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. PT8HALOZEO is a backbone containing the gene for the HALO tag, a promoter a zeomycin resistance gene and MCS (Multiple Cloning Site).
|Assay Target Type
Oncology & Inflammation