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HTRF PAb Anti-phospho ATF2-Eu cryptate, 500 Assay Points

PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.

Product Variants
Part number: 61P12KAE
Unit Size: 500 Assay Points
List price: USD 865.43
Your price:
USD 865.43
USD 865.43 /each
Part number: 61P12KAZ
Unit Size: 10,000 Assay Points
List price: USD 2,285.00
Your price:
USD 0.00
USD 2,285.00 /each


The Anti-phospho ATF2-Eu cryptate is a labeled polyclonal antibody enabling the detection of ATF2 when singly phosphorylated at Ser 71 or dually phosphorylated at Thr69/71. It does not detect the non-phosphorylated ATF2. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.


Assay Points
Assay Target Type
Fluorescent reagent
Assay Technology
Therapeutic Area
Infectious Diseases
Oncology & Inflammation
Rare Diseases
Unit Size
500 Assay Points

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How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.


HTRF PAb Anti-phospho ATF2-Eu cryptate 1.svg


Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho ATF2-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

HTRF PAb Anti-phospho ATF2-Eu cryptate 2a.svg



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HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, Manuals and more

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