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HTRF PAb Anti-phospho AKT substrate-Eu cryptate, 500 Assay Points

PAb Anti-phospho AKT substrate-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser/Thr in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.
Part Number: 61P02KAE
Unit Size: 500 Assay Points
Part Number: 61P02KAZ
Unit Size: 10,000 Assay Points
  • The Anti-phospho AKT substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of Akt when phosphorylated at Ser or Thr preceded by arginine or lysine in -5 and -3 positions. It does not cross-react with phosphorylated Ser or Thr in different configurations (slight cross reactions with -2 and +2 positions). It does not detect the non-phosphorylated Akt. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

  • Assay Points
    500
    Assay Target Type
    Fluorescent reagent
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Inflammation
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    500 Assay Points
  • Assay principle

    HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

    HTRF PAb Anti-phospho AKT substrate-Eu cryptate 1.svg

     

    Assay protocol

    HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho AKT substrate-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

    HTRF PAb Anti-phospho AKT substrate-Eu cryptate 2.svg

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.