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HTRF Mouse IL-17 Detection Kit, 10,000 Assay Points

The HTRF mouse IL17 kit is designed for the quantification of mouse IL17 release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62MIL17PEG
Unit Size: 500 Assay Points
Part Number: 62MIL17PEH
Unit Size: 10,000 Assay Points
  • IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of response to fungi and bacteria, and promotes release of or pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is main cytokine released by differentiated TH17 cells.

    Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

  • Assay Points
    10000
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    NASH/Fibrosis
    Oncology & Inflammation
    Unit Size
    10,000 Assay Points
  • Assay principle

    Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

    mouse-il17-kit

     

    Assay data analysis

    The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

  • Technical specifications of mouse IL17 kit
    Sample size 16 µL
    Final assay volume 20 µL
    Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
    LOd &LOQ (in Diluent) 4 pg/mL &16 pg/mL
    Range 16 – 4,000 pg/mL
    Time to result 1h at RT
    Species Mouse only

     

  • Intra and inter assay

    Intra-assay (n=24)

    Sample Mean [IL17] (pg/mL) CV
    1 25 18%
    2 1724 3%
    3 3448 4%
      Mean CV 8%

    Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

    Inter-assay (n=4)

    Sample [IL17] (pg/mL) Mean (delta R) CV
    1 41 273 5%
    2 256 1678 5%
    3 1600 9074 7%
        Mean CV 6%

    Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.