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HTRF Mouse IL-1α Detection Kit, 500 Assay Points

Mouse IL1-α kit is designed quantification of mouse IL1-α release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62MIL1APEG
Unit Size: 500 Assay Points
Part Number: 62MIL1APEH
Unit Size: 10,000 Assay Points
  • Also known as hematopoietin 1, IL1 alpha is a pro-inflammatory cytokine mainly produced by activated macrophages, and epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1 alpha stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Oncology & Inflammation
    Unit Size
    500 Assay Points
  • Assay principle

    Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

    mouse-il1-alpha-kit

     

    Assay data analysis

    The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

  • Technical specifications of mouse IL1 alpha kit
    Sample size 16 µL
    Final assay volume 20 µL
    Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
    LOD &LOQ (in Diluent) 34 pg/mL &62 pg/mL
    Range 62 – 4,000 pg/mL
    Time to result 3h at RT
    Calibration NIBSC (93/672) value (IU/mL) = 1,2 x HTRF mIL1a value (pg/mL)
    Species Mouse only

     

  • Intra and inter assay

    Intra-assay (n=24)

    Sample Mean [IL1a] (pg/mL) CV
    1 100 11%
    2 800 3%
    3 3200 1%
      Mean CV 5%

    Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

    Inter-assay (n=4)

    Sample [IL1a] (pg/mL) Mean (delta R) CV
    1 161 188 3%
    2 583 595 2%
    3 2105 1674 2%
        Mean CV 2%

    Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.