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HTRF MAb PY20-Eu cryptate, 5,000 Assay Points

MAb PY20-Eu cryptate enables the rapid detection of phosphorylation of peptides or proteins on Tyrosine in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.
Product Variants
Part number: 61Y20KLA
Unit Size: 5,000 Assay Points
List price: USD 408.06
Your price:
USD 408.06
USD 408.06 /each
Part number: 61Y20KLB
Unit Size: 20,000 Assay Points
List price: USD 1,176.00
Your price:
USD 0.00
USD 1,176.00 /each

Overview

PY20-Eu cryptate is a labeled mouse monoclonal IgG2b. It shows to react specifically with phosphorylated tyrosine, both as free amino acid or when conjugated to carriers. No cross-reactivity has been observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. This allows the construction of a highly specific biochemical Tyrosine kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.

Specifications

Assay Points
5000
Assay Target Type
Fluorescent reagent
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped Ambient
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
5,000 Assay Points

Video gallery

How it works

Assay principle

HTRF Tyrosine kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphotyrosine antibody, SA-XL665 and a biotinylated substrate. The signal is proportional to the concentration of phospho-residues. Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

Kinase how it works assay principle htrf kinase
Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb PY20-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.

Kinase how it works assay protocol htrf kinase

 

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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