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HTRF MAb Anti-phosphoSAP/JNK-Eu cryptate, 10,000 Assay Points

MAb Anti-phospho SAP/JNK-Eu cryptate enables the rapid detection of SAP/JNK phosphorylation on Thr183 &Tyr184 15 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.
Part Number: 61P09KAE
Unit Size: 500 Assay Points
Part Number: 61P09KAZ
Unit Size: 10,000 Assay Points
  • The Anti-phospho SAP/JNK-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of SAP/JNK only when phosphorylated at Thr183 & Tyr185. It does not cross-react with phosphorylated forms of p44/42 or p38 MAPK. It does not detect the non-phosphorylated SAP/JNK. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

  • Assay Points
    10000
    Assay Target Type
    Fluorescent reagent
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Inflammation
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    10,000 Assay Points
  • Assay principle

    HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

    HTRF MAb Anti-phospho SAPJNK-Eu cryptate 1.svg

     

    Assay protocol

    HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phosphoSAP/JNK-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

    HTRF MAb Anti-phospho SAPJNK-Eu cryptate 2.svg

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.