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HTRF MAb Anti-phospho AKT-Eu cryptate, 500 Assay Points

MAb Anti-phospho AKT-Eu cryptate enables the rapid detection of Akt phosphorylation on Ser473 in a biochemical kinase assay format.

For research use only. Not for use in diagnostic procedures.
Product Variants
Part number: 61P15KAE
Unit Size: 500 Assay Points
List price: USD 844.44
Your price:
USD 844.44
USD 844.44 /each
Part number: 61P15KAZ
Unit Size: 10,000 Assay Points
List price: USD 2,285.00
Your price:
USD 0.00
USD 2,285.00 /each

Overview

The Anti-phospho AKT-Eu cryptate is a labeled monoclonal antibody enabling the rapid detection of Akt only when phosphorylated at Ser473. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Specifications

Assay Points
500
Assay Target Type
Fluorescent reagent
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

HTRF MAb Anti-phospho AKT-Eu cryptate 1.svg

 

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and MAb Anti-phospho AKT-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.

HTRF MAb Anti-phospho AKT-Eu cryptate 2.svg

 

Resources

1-2 of 2 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

Application Note Icon Image
Application Note
TCR phospho signaling investigation with HTRF

Study your pathway of interest in PBMC and T cells

Combine models with a technique that maximizes each one’s relevance: this note...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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