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HTRF KinEASE-TK Detection Kit, 1,000 Assay Points

The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The TK kit uses a specific substrate compatible with tyrosine kinases - cf. validation data provided on this page.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 62TK0PEB
Unit Size: 1,000 Assay Points
List price: USD 1,095.00
Your price:
USD 1,095.00
USD 1,095.00 /each
Part number: 62TK0PEC
Unit Size: 20,000 Assay Points
List price: USD 5,040.00
Your price:
USD 0.00
USD 5,040.00 /each

Overview

The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE TK assay has been validated on 71 Tyrosine kinases, using a single generic substrate. The assay validation section of this page lists them and provides technical tips for setup.

Specifications

Assay Points
1000
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped Ambient
Therapeutic Area
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
Unit Size
1,000 Assay Points

Video gallery

How it works

Assay principle

Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. The TK generic substrate is made of a sequence of repetitive motifs recognized by most tyrosine kinases, and by the specific conjugated antibody once phosphorylated.

Assay protocol

As shown here, all HTRF KinEASE assays work the same way and involve two steps:

Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.

Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.

kinase-how-it-works-assay-principle-htrf-kinease

 

Resources

1-2 of 2 Resources
Literature - Publication Review
Download your literature review on the multiple use of KinEase assay kits

Discover the cutting edge of kinase assay knowledge

This literature review illustrates the possibilities and versatility of HTRF™...

Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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