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HTRF KinEASE-STK S2 Detection Kit, 1,000 Assay Points

The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The STK2 kit uses a specific substrate compatible with various Serine/thronine kinases - cf. validation data provided on this page.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 62ST2PEB
Unit Size: 1,000 Assay Points
List price: USD 1,107.00
Your price:
USD 1,107.00
USD 1,107.00 /each
Part number: 62ST2PEC
Unit Size: 20,000 Assay Points
List price: USD 7,343.00
Your price:
USD 0.00
USD 7,343.00 /each

Overview

The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits, offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE STK S2 assay has been validated on 18 Serine/Threonine kinases. The assay validation section of this page lists them and provides technical tips for setup. The kit series also include a Discovery kit to help determine which of three STK kits is appropriate when working with a non-validated enzyme.

Specifications

Assay Points
1000
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped Ambient
Therapeutic Area
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
Unit Size
1,000 Assay Points

Video gallery

References

How it works

Assay principle

Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. All three STK peptidic substrates are made of the same sequence for antibody detection, next to a variable sequence recognized by enzymes. The targeted residue for phosphorylation lies in between as part of both sequences for enzymatic activity (unphosphorylated) and antibody detection (phosphorylated). It has been shown that 3 different variable sequences could enable the study of most Serine/Threonine kinases.


Assay protocol

As shown here, all HTRF KinEASE assays work the same way and involve two steps:

Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.

Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.

kinase-how-it-works-assay-principle-htrf-kinease

 

Resources

1-3 of 3 Resources
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Literature - Publication Review
Download your literature review on the multiple use of KinEase assay kits

Discover the cutting edge of kinase assay knowledge

This literature review illustrates the possibilities and versatility of HTRF™...

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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Guide
The ultimate guide to successful kinase binding experiments

Your guide for mastering the art of kinase binding assay optimizations

This complete guide provides you with all the tools you need...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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