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HTRF Hybridoma binding (GST antigen) Kit, 500 Assay Points

The hybridoma binding (GST antigen) kit is designed to screen mouse hybridoma supernatants against GST-tagged targets.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 5 µL

The hybridoma binding (GST antigen) kit is designed to screen mouse hybridoma supernatants against GST-tagged targets.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
64HYGSTPEG
List Price
USD 956.69
Unit Size: 10,000 Assay Points
Part #:
64HYGSTPEH
List Price
USD 12,700.00

Overview

The hybridoma technology is still the most common technique used to generate antibodies for therapeutic applications. The generation and screening of hybridomas from an immunized animal involve a time-consuming process and sample only a fraction of the antibodies generated during the adaptive immune response. Therefore, screening hybridoma supernatants for their ability to efficiciently bind the target of interest has become crucial.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Binding Assay
Technology
TR-FRET
Therapeutic Area
Inflammation
Neuroscience
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay Principle

The mouse hybridoma supernatant binding to a GST-tagged antigen is measured using a sandwich immunoassay, involving an anti-GST antibody labeled with an HTRF donor and an anti-mouse antibody labeled with an acceptor dye. The binding of murine antibodies produced by the hybridoma clone to their GST-tagged target generates an HTRF Signal.

hybridoma-binding-kit

 

Assay Protocol

The HTRF hybridoma binding kit can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples are dispensed directly into the assay plate. The GST antigen is then added, followed by the dispensing of the HTRF reagents: the Anti GST antibody labeled with europium cryptate and the anti mouse antibody labeled with d2. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

hybridoma-binding-kit

 

Assay validation

GST tagged beta catenin binding assay

Binders of Beta catenin fused to the GST tag was detected using an anti GST europium cryptate antibody and an anti mouse d2 antibody.

The hybridoma binding kit protocol was performed using 5 nM GST tagged Beta catenin protein for 1H at room temperature.

The graph shows the signal obtained with a positive sample (red) and a negative sample (orange).

The Hybridoma (GST antigen) control was also used to validate the experiment.

hybridoma-binding-kit

 

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