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HTRF Hybridoma binding (GST antigen) Kit, 500 Assay Points

The hybridoma binding (GST antigen) kit is designed to screen mouse hybridoma supernatants against GST-tagged targets.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 64HYGSTPEG
Unit Size: 500 Assay Points
Part Number: 64HYGSTPEH
Unit Size: 10,000 Assay Points
  • The hybridoma technology is still the most common technique used to generate antibodies for therapeutic applications. The generation and screening of hybridomas from an immunized animal involve a time-consuming process and sample only a fraction of the antibodies generated during the adaptive immune response. Therefore, screening hybridoma supernatants for their ability to efficiciently bind the target of interest has become crucial.

  • Assay Points
    500
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Inflammation
    Neuroscience
    Oncology & Inflammation
    Unit Size
    500 Assay Points
  • Assay Principle

    The mouse hybridoma supernatant binding to a GST-tagged antigen is measured using a sandwich immunoassay, involving an anti-GST antibody labeled with an HTRF donor and an anti-mouse antibody labeled with an acceptor dye. The binding of murine antibodies produced by the hybridoma clone to their GST-tagged target generates an HTRF Signal.

    hybridoma-binding-kit

     

    Assay Protocol

    The HTRF hybridoma binding kit can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples are dispensed directly into the assay plate. The GST antigen is then added, followed by the dispensing of the HTRF reagents: the Anti GST antibody labeled with europium cryptate and the anti mouse antibody labeled with d2. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

    hybridoma-binding-kit

     

  • GST tagged beta catenin binding assay

    Binders of Beta catenin fused to the GST tag was detected using an anti GST europium cryptate antibody and an anti mouse d2 antibody.

    The hybridoma binding kit protocol was performed using 5 nM GST tagged Beta catenin protein for 1H at room temperature.

    The graph shows the signal obtained with a positive sample (red) and a negative sample (orange).

    The Hybridoma (GST antigen) control was also used to validate the experiment.

    hybridoma-binding-kit

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.