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HTRF XIAP BIR3 Binding Kit, 500 Assay Points

The XIAP BIR3 Binding Kit is designed to identify, characterize, and profile compounds that bind to the BIR3 domain of the E3 ligase XIAP protein.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 64XIAPB3PEJ
Unit Size: 100,000 Assay Points
Part Number: 64XIAPB3PEG
Unit Size:
Part Number: 64XIAPB3PEH
Unit Size:
  • A fast and easy way to identify and characterize new binders to XIAP protein targeting the BIR3 domain.

    X-linked inhibitor of apoptosis protein (XIAP) protein belongs to the inhibitor of apoptosis protein (IAP) family, which also includes cIAP1. It is the most potent caspase inhibitory IAP family member and a negative regulator of various apoptotic stimuli and death pathways.

    XIAP overexpression in tumor cells is a well-described mediator of resistance to chemotherapy in many cancers and has been linked to tumor aggressiveness, making it an attractive target in cancer therapy where several therapeutic strategies have been investigated, such as SMAC mimetics.

    Moreover, XIAP displays E3 ligase activity and leads to targeted proteins' ubiquitination and their subsequent degradation. This property can be exploited through a Proteolysis-targeting chimera (PROTAC) strategy. Therefore, new compounds targeting XIAP which exhibit dual roles: i) inhibition of XIAP anti-apoptotic function and ii) induction of targeted protein degradation, represent a promising therapeutic approach.

    XIAP, like other IAP family proteins, contains BIR domains which interact with the IAP-binding motif of partners such as caspases. This interaction has been suggested to control pro- and anti-apoptotic activities.

    XIAP compound characterization on the BIR2 and BIR3 domains enables accurate profiling and selectivity studies.

  • Application
    Protein Detection
    Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Automation Compatible
    Yes
    Brand
    HTRF
    Detection Method
    HTRF
    Experimental Type
    In vitro
    Quantity
    1
    Therapeutic Area
    Inflammation
    Neuroscience
    Oncology & Inflammation

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