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HTRF Human IL-5 Detection Kit, 10,000 Assay Points

The HTRF human IL5 kit is designed for the quantification of human IL5 release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62HIL05PEG
Unit Size: 500 Assay Points
Part Number: 62HIL05PEH
Unit Size: 10,000 Assay Points
  • IL5 is mainly produced by TH2 cells upon IL4 activation, but also by eosinophils and mast cells. IL5 promotes B cell growth and stimulates immunoglobulin secretion. It also induces eosinophil activation, proliferation, and differentiation.

    Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

  • Assay Points
    10000
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Oncology & Inflammation
    Unit Size
    10,000 Assay Points
  • Assay principle

    Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

    cytokines-how-it-works-assay-principle-human
    Assay data analysis

    The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

    Revvity also worked with Myassays.com to help you in your data analysis.

     

  • Technical specifications of human IL5 kit
    Sample size 16 µL
    Final assay volume 20 µL
    Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol
    LOD &LOQ (in Diluent) 15 pg/mL &71 pg/mL
    Range 71 20,000 pg/mL
    Time to result 2h at RT
    Calibration NIBSC (90/586) value (IU/mL) = 0,012 x HTRF hIL5 value (pg/mL)
    Species Human only

     

  • Intra and inter assay

    Intra-assay (n=24)

    Sample Mean [IL5] (pg/mL) CV
    1 104 9%
    2 969 2%
    3 8064 2%
      Mean CV 4%


    Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

    Inter-assay (n=4)

    Sample [IL5] (pg/mL) Mean (delta R) CV
    1 388 552 4%
    2 1878 2413 4%
    3 4131 5060 4%
        Mean CV 4%


    Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.