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HTRF Human IgG Kit, 500 Assay Points

The Human IgG kit is designed for the fast quantification of human IgG.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6FHIGPEG
Unit Size: 500 Assay Points
Part Number: 6FHIGPEH
Unit Size: 10,000 Assay Points
  • Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Rare Diseases
    Unit Size
    500 Assay Points
  • Assay principle

    This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2. The signal intensity is proportional to the number of antibody-antibody complexes formed and therefore to the human IgG concentration.

    Biomarkers how it works assay principle human
    Assay Protocol

    The human IgG assay features a streamlined protocol with only 1 incubation step after sample and human IgG detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

    Biomarkers assay protocol human

     

  • Key features

    The figure below shows the human IgG kit calibration curve. The assay was performed in a 384-well small volume white microplate in a 20 µL total volume, using 10µL human IgG calibrator diluted in PBS 0.1 M and 5 µL of each conjugate. The two HTRF conjugates may be pre-mixed and added in a single dispensing step to further streamline the protocol. After 2h of incubation, the signal was read on a PHERAstarFS flash lamp.

    Detection limit 0.9 ng/mL
    Assay range 0.9 to 2000 ng/mL
    Incubation time 2h at RT
    Specificity IgG1, IgG2, IgG3, IgG4
    Specifications human igg

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.