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HTRF Human GM-CSF Detection Kit, 500 Assay Points

The HTRF human GM-CSF kit is designed for the quantification of human GM-CSF release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 62HGMCSFPEG
Unit Size: 500 Assay Points
List price: USD 1,370.00
Your price:
USD 1,370.00
USD 1,370.00 /each
Part number: 62HGMCSFPEH
Unit Size: 10,000 Assay Points
List price: USD 14,590.00
Your price:
USD 0.00
USD 14,590.00 /each

Overview

Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony-stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endolial cells, and fibroblasts. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

cytokines-how-it-works-assay-principle-human-gm-csf-62hgmcsfpeg-62hgmcsfpeh.svg
Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Revvity also worked with myassays.com to help you in your data analysis. 
 

Assay details

Technical specifications of human GM-CSF kit
Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 27 pg/mL & 47 pg/mL
Range 47 – 4,500 pg/mL
Time to result Overnight at RT
Calibration NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF
Species Human only

 

Analytical performance

Intra and inter assay

Intra assay (n=24)

Sample Mean [GM-CSF] (pg/mL) CV
1 81 17%
2 2125 4%
3 6011 10%
  Mean CV 10%

 

Inter assay (n=4)

Sample [GM-CSF] (pg/mL) Mean (delta ratio)  
1 169 370 6%
2 774 1654 7%
3 4135 5313 6%
    Mean CV 7%

 

Assay validation

GM-CSF secretion by PBMC co-stimulated with ionomycin and PMA

PBMCs, plated at 100,000 cells/well in a 96 well plate, were stimulated for 24h with 1µg/ml of ionomycin and with various concentrations of PMA. 16µl of supernatant were transferred into a 384 well (small volume) white detection plate to be analyzed by the Human GM-CSF Assay.

assay-validation-human-gm-csf-1.svg

 

Resources

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Guide
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SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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