HTRF Human GM-CSF Detection Kit, 500 Assay Points

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HTRF Human GM-CSF Detection Kit, 500 Assay Points

The HTRF human GM-CSF kit is designed for the quantification of human GM-CSF release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

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The HTRF human GM-CSF kit is designed for the quantification of human GM-CSF release in cell supernatant.

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Product Variants

Unit Size: 500 Assay Points

Part #:

62HGMCSFPEG

List Price

USD 1,370.00

Unit Size: 10,000 Assay Points

Part #:

62HGMCSFPEH

List Price

USD 14,590.00

Product information

Overview

Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony-stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endolial cells, and fibroblasts. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Cytokines
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Oncology & Inflammation
Unit Size	500 Assay Points

Video gallery

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Revvity also worked with myassays.com to help you in your data analysis.

Assay details

Technical specifications of human GM-CSF kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent)	27 pg/mL & 47 pg/mL
Range	47 – 4,500 pg/mL
Time to result	Overnight at RT
Calibration	NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF
Species	Human only

Analytical performance

Intra and inter assay

Intra assay (n=24)

Sample	Mean [GM-CSF] (pg/mL)	CV
1	81	17%
2	2125	4%
3	6011	10%
	Mean CV	10%

Inter assay (n=4)

Sample	[GM-CSF] (pg/mL)	Mean (delta ratio)
1	169	370	6%
2	774	1654	7%
3	4135	5313	6%
		Mean CV	7%

Assay validation

GM-CSF secretion by PBMC co-stimulated with ionomycin and PMA

PBMCs, plated at 100,000 cells/well in a 96 well plate, were stimulated for 24h with 1µg/ml of ionomycin and with various concentrations of PMA. 16µl of supernatant were transferred into a 384 well (small volume) white detection plate to be analyzed by the Human GM-CSF Assay.