Skip to main content

HTRF Human Fc Detection Kit, 500 Assay Points

The Human Fc kit is designed for the fast quantification of human IgG or Human Fc tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62HFCPEG
Unit Size: 500 Assay Points
Part Number: 62HFCPEH
Unit Size: 10,000 Assay Points
  • Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human Fc kit enables a rapid quantification of human IgG, and any proteins containing a human Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Shipping Conditions
    Shipped Ambient
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Rare Diseases
    Unit Size
    500 Assay Points
  • Assay principle

    The human Fc kit is a competitive immunoassay for the measurement of hIgGs or any hFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to hFc and d2 coupled hIgGs. hFc provided by the sample competes with d2-labeled human IgG for binding to anti-human Fc Eu Cryptate.

    assay-principle-human
    Assay Protocol

    The human Fc assay features a streamlined protocol with only 1 incubation step after sample and Human Fc detection reagents dispensing. This protocol requires a single 2-and-a-half-hour incubation period at RT.

    biomarkers-how-it-works-assay-protocol-human

     

  • Key features
    Detection limit: 9 ng/mL
    Dynamic range: 5.5 to 4,000 ng/mL
    Incubation time: 2h30 to ON
    EC50: 216 - 296 ng/mL
    specifications-human-fc

     

  • Whole hIgG and Fc fragment detection

    Human Fc chimeric antibodies as well as proteins containing a human Fc region can be detected using the HTRF human Fc assay. As shown in Figure 1, the affinity depends on the nature of each Fc chimera and is at its maximum for whole IgG. This means that the assessment of the concentration can be made on a relative basis using the human IgG calibrator. Absolute concentration can be approached if the calibrator is made with the same protein as that being assessed.

    Figure 1: Various concentrations of four different human Fc chimeras were tested using the Total human IgG assay, following standard assay protocols.

    assay-validation-human-fc
    Media compatibility

    Figure 2 demonstrates the compatibility of the HTRF human Fc assay with different media, even in the presence of 10% FCS. No impact on assay performance was seen. Unlike other immunoassays involving streptavidin-biotin detection, this assay is insensitive to the presence of culture medium, whose quenching effects can be compensated for by our patented 665/620 ratio.

    Figure 2: IgG standard curve was drawn up using IgG in phosphate buffer, culture media and RPMI containing 10% FCS+/- 4.4% PSGHT (Penicillin, Streptamycin, Glutamine, Hypoxanthine, Thymidine).

    assay-validation-human-fc

     

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.