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HTRF Human TNFα/TNFR1 Binding Kit, 500 Assay Points

The Human TNFalpha/TNFR1 binding kit is designed to identify human TNFalpha/TNFR1 inhibitors.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: 64BDTNFPEG
Unit Size: 500 Assay Points
List price: USD 1,254.00
Your price:
USD 1,254.00
USD 1,254.00 /each
Part number: 64BDTNFPEH
Unit Size: 10,000 Assay Points
List price: USD 15,010.00
Your price:
USD 0.00
USD 15,010.00 /each

Overview

Deregulation of the tumor necrosis factor (TNF) plays an important role in the

initiation and perpetuation of chronic inflammation and has been implicated in the development of various autoimmune diseases. TNFalpha inhibitors are successfully used for the treatment of several diseases, such as rheumatoid arthritis and psoriasis.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The HTRF TNFα/TNFR1 Binding kit is designed to measure the interaction between TNFα and TNFR1 proteins. Without treatment, TNFa binds to TNFR1, and the binding of each detection antibody to its respective tagged protein generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the TNFa/TNFR1 interaction is disrupted and the HTRF signal decreases.

human-TNFalpha-TNFR1-binding-kit-1.svg

 

Assay protocol

The Human TNFalpha/TNFR1 binding kit can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, samples or standards are dispensed directly into the assay plate. The Tag1-TNFalpha protein and Tag2-TNFR1 protein are then added, followed by the dispensing of the HTRF reagents: the Tag1 antibody labeled with europium cryptate and the Tag2 XL665 reagent. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.

 

human-TNFalpha-TNFR1-binding-kit-2.svg

 

Assay validation

Inhibitory effects of TNFalpha/TNFR1 blockers

The inhibitory effects of small molecule unlabelled TNFR1 and TNFalpha, human anti-TNFalpha antibody (Adalimumab), and mouse anti-TNFR1 were tested. No inhibition was observed using an anti TNFR2 antibody, confirming the specificity of the kit for the study of TNFalpha/TNFR1 interaction.

human-TNFalpha-TNFR1-binding-kit-3.svg

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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