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HTRF Cynomolgus TNFα Detection Kit, 500 Assay Points

The HTRF cynomolgus TNF alpha kit is intended for the quantification of cynomolgus TNF alpha release in cell supernatant.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 62CTNFAPEG
Unit Size: 500 Assay Points
List price: USD 1,103.00
Your price:
USD 1,103.00
USD 1,103.00 /each
Part number: 62CTNFAPEH
Unit Size: 10,000 Assay Points
List price: USD 10,290.00
Your price:
USD 0.00
USD 10,290.00 /each

Overview

TNF alpha, mainly produced by activated macrophages, is one of main cytokines in acute phase reaction. Depending on cellular target and biological context, TNF alpha can activate pro-apoptosis or pro-survival pathways, thus regulating tissue development through apoptosis control. Outside of oncology and inflammation, TNF alpha contributes to development of type II diabetes by increasing insulin resistance and modulating fatty acid metabolism.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Therapeutic Area
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
Unit Size
500 Assay Points

Video gallery

References

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Cytokines-how-it-works-assay-principle-cynomolgus-tnf-alpha-62ctnfapeg
Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Assay details

Technical specifications of Cynomolgus TNF alpha
Sample size 16 µL
Final assay volume  20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol
LOD & LOQ (in Diluent) 12.4 pg/mL & 27 pg/mL
Range 27 – 3,000 pg/mL
Time to result ON at RT
Species Cynomolgus only

 

Analytical performance

Inter and Intra assay
Sample Mean [TNFa] (pg/mL) CV
1 100 14%
2 1500 2%
3 2750 2%
  Mean CV 6%

 

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Sample [TNFa] (pg/mL) Mean (delta R) CV
1 159 665 3%
2 514 2150 2%
3 1667 6467 2%
    Mean CV 2%

 

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

 

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, Manuals and more

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