US
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
PHOTO CYNOMOLGUS TNF ALPHA KIT
The HTRF cynomolgus TNF alpha kit is intended for the quantification of cynomolgus TNF alpha release in cell supernatant.
Click to copy promo code to clipboard.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
The HTRF cynomolgus TNF alpha kit is intended for the quantification of cynomolgus TNF alpha release in cell supernatant.
Click to copy promo code to clipboard.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
Product Variants
Unit Size: 500 assay points
Part #:
62CTNFAPEG
List Price
USD 1,149.33
Your online price:
Unit Size: 10,000 assay points
Part #:
62CTNFAPEH
List Price
USD 10,722.18
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
PHOTO CYNOMOLGUS TNF ALPHA KIT
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
Product information
Overview
TNF alpha, mainly produced by activated macrophages, is one of main cytokines in acute phase reaction. Depending on cellular target and biological context, TNF alpha can activate pro-apoptosis or pro-survival pathways, thus regulating tissue development through apoptosis control. Outside of oncology and inflammation, TNF alpha contributes to development of type II diabetes by increasing insulin resistance and modulating fatty acid metabolism.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Cynomolgus
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
HTRF Cynomolgus TNF-α Detection Kit, 500 Assay Points
Citations
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Assay details
Technical specifications of Cynomolgus TNF alpha
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol |
| LOD & LOQ (in Diluent) | 12.4 pg/mL & 27 pg/mL |
| Range | 27 – 3,000 pg/mL |
| Time to result | ON at RT |
| Species | Cynomolgus only |
Analytical performance
Inter and Intra assay
| Sample | Mean [TNFa] (pg/mL) | CV |
|---|---|---|
| 1 | 100 | 14% |
| 2 | 1500 | 2% |
| 3 | 2750 | 2% |
| Mean CV | 6% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
| Sample | [TNFa] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 159 | 665 | 3% |
| 2 | 514 | 2150 | 2% |
| 3 | 1667 | 6467 | 2% |
| Mean CV | 2% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Resources
Are you looking for resources, click on the resource type to explore further.
Brochure
HTRF assays and reagents catalog
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
How can we help you?
We are here to answer your questions.
