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HTRF CD64 (FcgRI) Cellular Binding Kit, 500 Assay Points

The CD64 cellular binding kit is designed for monitoring the binding of the IgG Fc region to the human CD64 receptor on live cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6FC64PAG
Unit Size: 100 Assay Points
Part Number: 6FC64PAE
Unit Size: 500 Assay Points
Part Number: 6FC64PAF
Unit Size: 1,000 Assay Points
  • The CD64 (FcgRI) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD64 receptor in live cells and to select the most potent antibody for use in protective functions of the immune system. This assay, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting phagocytosis and immune cell activation.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Rare Diseases
    Unit Size
    500 Assay Points
  • Assay principle

    The CD64 receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD64 variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabeled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.

    Assay principle
    Assay protocol

    The CD64 receptor cellular binding assays (FcgI) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.

    Biomarkers how it works assay protocol

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.