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HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 62AM6PEB
Unit Size: 1,000 Assay Points
Part Number: 62AM6PEC
Unit Size: 20,000 Assay Points
  • Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.

    Revvity's HTRF cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity

  • Assay Points
    20000
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    20,000 Assay Points
  • Assay principle

    Revvity cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.

    GPCR how it works assay principle camp gs hirange
    Assay protocol

    The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents.

    GPCR how it works assay protocol camp gs hirange 62am6peb

     

  • cAMP Gs hirange product specifications

    If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!

    The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.

    Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.

     

    Specifications camp gs hirange 1

     

      S/B IC20 nM IC50 nM IC80 nM
    cAMP - Gs dynamic >14 0.91 4.07 18.1
    cAMP - Gs HiRange >28 4.27 22.8 121

     

    ATP cross-reactivity
    specifications-camp-gs-hirange-2.svg
    Specifications camp gs hirange 3

     

    Specifications camp gs hirange 4

     

     

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.