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HTRF α-Synuclein Aggregation Detection Kit, 500 Assay Points

This HTRF kit is designed for an accurate quantitative measurement of alpha synuclein aggregation in brain extracts.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 6FASYPEG
Unit Size: 500 Assay Points
List price: USD 1,603.00
Your price:
USD 1,603.00
USD 1,603.00 /each
Part number: 6FASYPEH
Unit Size: 10,000 Assay Points
List price: USD 18,890.00
Your price:
USD 0.00
USD 18,890.00 /each

Overview

Monitoring alpha-synuclein aggregation is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson's disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA). The kit provides a solution to rapidly detect and quantify protein aggregates of human alpha-synuclein in cell culture or brain tissue extracts.

Specifications

Assay Points
500
Assay Target Type
Kit
Assay Technology
HTRF
Brand
HTRF
Quantity
1
Therapeutic Area
Neuroscience
Unit Size
500 Assay Points

Video gallery

References

How it works

Assay principle

Alpha-Synuclein aggregates are measured using a sandwich immunoassay involving an anti-a Synuclein monoclonal antibody specific for alpha-Synuclein aggregate labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the alpha-Synuclein aggregates.

biomarkers-how-it-works-assay-principle-alpha-synuclein-aggregation-6fasypeg-6fasypeh
Assay Protocol

The sample or brain extracts are transferred to the assay plate for the detection of a-Synuclein aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

biomarkers-how-it-works-assay-protocol-alpha-synuclein-aggregation-6fasypeg-6fasypeh

 

Assay validation

Alpha synuclein aggregation in human brain samples

Samples from human putamen (Parkinson's versus control) were collected and lysed with a Revvity lysis buffer. Due to the nature of the samples, the conventional cellular protocol needed to be adjusted. Incubation was performed for 20 hours at RT. Even at high dilution (1/64), clear discrimination between the two sample types is possible with Revvity's aggregated alpha synuclein assay.

assay-validation-alpha-synuclein-aggregation-1

 

Resources

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HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, Manuals and more

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