GrowDex has been designed to provide a support matrix for the culture of cells in a wide range of applications. It comprises of two components, nanofibrillar cellulose (NFC) and ultra-pure water. The length of the nanofibrils ranges from 200-300 μm, with a diameter in the nanometer scale varying from 5-100 nm.

The product is supplied sterilized, ready to use and can be utilized in a variety of cell culture applications, such as 3D spheroid or organoid formation.

Benefits:

• Animal-free: No animal biomolecules interfering with readouts.
• Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
• Reliable: robust and sustained performance regardless of the production lot ordered.
• Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
• Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
• Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
• Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

• Storage: Ambient
• Shelf life: 12 months
• Shelf life after opening: 3 months

Concentration

• GrowDex is supplied at a concentration of 1.5%.
• Working concentrations are usually between 0.2% and 1.0%.

GrowDase™ enzyme breaks down GrowDex hydrogel to form a solution in an easy one step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.