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PhenoVue Hoechst 33342 Nuclear Stain 10mg

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only, not for use in diagnostic procedures.

Product Variants
Part number: CP71
Unit Size: 1x10mg
List price: USD 58.13
Your price:
USD 58.13
USD 58.13 /each
Part number: CP72
Unit Size: 1x100mg
List price: USD 112.10
Your price:
USD 0.00
USD 112.10 /each


PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 540nm. It is commonly used to stain cells' nuclear compartment.

PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Numbers of vials per unit 1
Quantity or volume per vial 10mg​ (10 mg/mL - 16.23mM)​​
Form Liquid​
Storage -20°C
Recommended working concentration 2µg/mL (3.24µM)​
Maximum excitation wavelength 357 nm
Maximum emission wavelength 540 nm
Common filter set DAPI
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 170-520 x 96-well microplates
140-520 x 384-well microplates
270-800​ x 1536-well microplates



Organelle and Cell Compartment
Product Compatibility
Live and fixed samples
Shipping Conditions
Shipped Ambient
Individual Reagent
Unit Size

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1-5 of 5 Resources
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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Hoechst 33342 Nuclear Stain Product Information Sheet

This is a product information sheet for PhenoVue Hoechst 33342 Nuclear Stain.

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PhenoVue Reagents & Imaging Microplates Product List
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Application Note
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SDS, COAs, Manuals and more

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