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DELFI EuTDA Cytotoxicity Detection kit, 1 item

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AD0116
Part Number: AD0116
  • DELFIA time-resolved fluorescence cell cytotoxicity assays offer a non-radioactive method to Chromium 51, for use in cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity (ADCC) studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

    Reagents for the assay are also available separately:

    • Lysis buffer (30 mL), #4005-0010
    • DELFIA Eu-Solution (200 mL), #C135-100
    • DELFIA BATDA labeling reagent (50 µL), #C136-100

    Selected references:

    1. Lazar, G. A. et al. Engineered antibody Fc variants with enhanced effector function. PNAS 103, 4005-4010 (2006).
    2. Tsai, C.-Y. et al. Type I IFNs and IL-18 Regulate the Antiviral Response of Primary Human γδ T Cells against Dendritic Cells Infected with Dengue Virus. The Journal of Immunology 194, 3890-3900 (2015).
    3. Mehta, R. S., Chen, X., Antony, J., Boyiadzis, M. & Szabolcs, P. Generating Peripheral Blood Derived Lymphocytes Reacting Against Autologous Primary AML Blasts. J Immunother 39, 71-80 (2016).
    4. Snyder, K. M. et al. Expression of a Recombinant High Affinity IgG Fc Receptor by Engineered NK Cells as a Docking Platform for Therapeutic mAbs to Target Cancer Cells. Front Immunol 9, (2018).
    5. Yu, T., Qiao, C., Lv, M. & Tang, L. Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity. BMC Biotechnology 19, 28 (2019).
  • Automation Compatible
    Yes
    Brand
    DELFIA
    Detection Method
    DELFIA TRF
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Format
    Microplates

Resources

1-3 of 3 Resources
Application Note Icon Image
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development
BioTherapeutics, DELFIA, TRF, time-resolved fluorescence
Guide
DELFIA immunoassay development guide
DELFIA, guide, protocol, assay, immunoassay
Guide
Time-resolved fluorometry with the DELFIA method user guide
DELFIA, guide, fluorometry, assays, TRF technology