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DELFI EuTDA Cytotoxicity Detection kit, 1 item

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: AD0116
List price: USD 579.05
Your price:
USD 579.05
USD 579.05 /each

Overview

DELFIA time-resolved fluorescence cell cytotoxicity assays offer a non-radioactive method to Chromium 51, for use in cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity (ADCC) studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Reagents for the assay are also available separately:

  • Lysis buffer (30 mL), #4005-0010
  • DELFIA Eu-Solution (200 mL), #C135-100
  • DELFIA BATDA labeling reagent (50 µL), #C136-100

Selected references:

  1. Lazar, G. A. et al. Engineered antibody Fc variants with enhanced effector function. PNAS 103, 4005-4010 (2006).
  2. Tsai, C.-Y. et al. Type I IFNs and IL-18 Regulate the Antiviral Response of Primary Human γδ T Cells against Dendritic Cells Infected with Dengue Virus. The Journal of Immunology 194, 3890-3900 (2015).
  3. Mehta, R. S., Chen, X., Antony, J., Boyiadzis, M. & Szabolcs, P. Generating Peripheral Blood Derived Lymphocytes Reacting Against Autologous Primary AML Blasts. J Immunother 39, 71-80 (2016).
  4. Snyder, K. M. et al. Expression of a Recombinant High Affinity IgG Fc Receptor by Engineered NK Cells as a Docking Platform for Therapeutic mAbs to Target Cancer Cells. Front Immunol 9, (2018).
  5. Yu, T., Qiao, C., Lv, M. & Tang, L. Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity. BMC Biotechnology 19, 28 (2019).

Specifications

Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 Kit

Resources

1-3 of 3 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide Icon
Guide
DELFIA immunoassay development guide

DELFIA immunoassays are a superior alternative to traditional ELISAs

Guide Icon
Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

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