Skip to main content

DELFIA BATDA, 50 µL

BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part number: C136-100
List price: USD 264.35
Your price:
USD 264.35
USD 264.35 /each

Overview

The DELFIA® BATDA Reagent is intended for labeling of cells to be used in short term cytotoxicity tests performe utilizing time-resolved fluorometry in the detection. It forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA BATDA labeling reagent is sufficient for 10x96 wells.

DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Specifications

Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped in Blue Ice
Unit Size
50 µL

Resources

1-2 of 2 Resources
Application Note Icon Image
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon