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LANCE Ultra Anti-2-methyl K9 Histone H3 Antibody, 15,625 Assay Points

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF0403-D
Unit Size: 10 µg
Part Number: TRF0403-M
Unit Size: 100 µg
  • The LANCE® Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2) antibody was used for the development and optimization of a G9a histone H3 methyltransferase assay using a biotinylated Histone H3 (1-21) peptide as substrate. A technical note describing the assay is available in our product literature.


    • 10 µg = 1,500 assay points (384-well assay format)
    • 100 µg = 15,000 assay points (384-well format)

    Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

    Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

  • Antibody Conjugates
    Automation Compatible
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    Experimental Type
    In vitro
    Molecular Modification
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Unit Size
    100 µg