ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Viability |
Assay Points | 100 |
ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.
Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
Features and benefits:
An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.
Application |
Cell Viability
|
---|---|
Assay Points |
100
|
Automation Compatible |
Yes
|
Brand |
ATPlite 1step
|
Cellular or Signaling Pathway |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
Cellular Process |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
Detection Modality |
Luminescence
|
Format |
Microplates
|
Protocol |
1-step
|
Signal Halflife |
0.5 hour
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
10 mL
|
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