Luciferase Assay Systems

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Luciferase assays are a subset of luminescence assays that evaluate ATP levels or gene expression by measuring the light generated by luciferase enzymes.

It allow for the study of transcriptional gene expression, virus life cycles, and cell viability, making them significant tools for drug development in reporter gene, cytotoxicity, and cell proliferation applications. Revvity's luciferase-based luminescence assays include reporter gene and ATP-monitoring.

Advantages of Luciferase Assays as a Detection Platform

A luminescence assay is extremely useful as a detection platform for several reasons, including:

Wide dynamic range
Greater sensitivity than fluorescence technologies
Lower interference than other detection options
Cost effective detection
Simple automation for high throughput applications
Homogenous luciferase assay set-up eliminates wash and separation steps

Our luciferase luminescence assay options allow high sensitivity in a convenient microplate format.

Discover our latest innovation: ATPlite 1glow. This single-addition assay offers extended signal stability (>3 hours), a broad dynamic range, and seamless integration into high-throughput automation workflows. Available in both liquid and lyophilized formats.

For research use only. Not for use in diagnostic procedures.

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Reporter Gene Assays

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells.

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Cytotoxicity and Cell Proliferation Assays

Cytotoxicity and cell proliferation assays are commonly used in the drug discovery process to assess a compound's ability to cause or block a biologic activity without having toxic effects on cells. Adenosine TriPhosphate (ATP) – monitoring assays allow for the quantitative evaluation of proliferation and cytotoxicity.

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