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ATPlite 1step Luminescence Assay System, 100 mL ATP Assay Kit

ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: 6016736
Unit Size: 10 mL
List price: USD 87.28
Your price:
USD 0.00
USD 87.28 /each
Part number: 6016731
Unit Size: 100 mL
List price: USD 646.26
Your price:
USD 646.26
USD 646.26 /each
Part number: 6016739
Unit Size: 1,000 mL
List price: USD 4,334.00
Your price:
USD 0.00
USD 4,334.00 /each

Overview

ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.

Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

Features and benefits:

  • Simple and reproducible: only one reagent addition step, no separation steps
  • Suitable for both 96- and 384-well microplates
  • Linear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplates
  • Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shaking
  • Homogeneous: no cell harvesting or centrifugation required
  • High sensitivity: able to detect down on one cell per well
  • Reduced Phenol Red dependency
  • High light-output: assay can be used with less sensitive luminescence readers such as multi-label readers
  • Fast: no luminescence signal stabilization time required

An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.

Specifications

Assay Points
1000
Assay Protocol
1-step
Automation Compatible
Yes
Brand
ATPlite
Cellular Process
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Detection Method
Luminescence
Experimental Type
In vitro
Format
Microplates
Shipping Conditions
Shipped Ambient
Unit Size
100 mL

References

Resources

1-5 of 5 Resources
Application Note Icon
Application Note
ATPlite assay performance in human primary cells.

In this application note three ATP monitoring luminescence assays were assessed and compared head-to-head: (i) ATPlite™ from...

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Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer

AlphaLISA and LANCE (TR-FRET) biomarker assays can be used to measure ECM-associated protein modulation caused by human...

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Application Note
Multi-parametric assessment of EGF treatment effects on signaling pathways, growth and proliferation using AlphaLISA SureFire Ultra and cellular imaging

In this application note, we demonstrate an efficient cell-based workflow for the assessment of EGF treatment effects in a...

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Application Note
Proliferation and cell death analyses of 3D cultures using Revvity CellCarrier Spheroid ULA microplates and ATPlite 3D products.

In the process of developing new therapeutic molecules, toxicity – whether it is to be avoided or whether it is desired – is an...

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Application Note
Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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