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AlphaLISA Mouse TREM2 Detection Kit, 5,000 Assay Points

The AlphaLISA® mouse TREM2 Detection Kit is designed for detection and quantitation of mouse TREM2 in buffer, cell culture media, serum, plasma, cerebrospinal fluid and cell supernatant using a homogeneous AlphaLISA assay (no wash steps).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL591HV
Unit Size: 100 Assay Points
Part Number: AL591C
Unit Size: 500 Assay Points
Part Number: AL591F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 μL reaction volume (10 μL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

    AlphaLISA features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA® technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

    TREM2 is a transmembrane molecule expressed on myeloid cells. It acts as the receptor for an unknown ligand to activate myeloid cells such as dendritic cells, increasing phagocytic activity. Recently, TREM2 has been shown to be involved in neurodegenerative diseases such as ataxia, early dementia and Alzeimer’s disease. Elevated levels of TREM2 have been noted in cerebrospinal fluid as a response to Alzeimer’s disease.

  • Application
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target Class
    Protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Species
    Mouse
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Neuroinflammation
    Unit Size
    5,000 Assay Points

Resources

1-3 of 3 Resources
Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

Guide
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

Application Note Icon Image
Application Note
Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...