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AlphaLISA IP-One Detection Kit, 1,000 Assay Points

The AlphaLISA® IP-One Kit is a competitive immunoassay for quantitative detection of IP1 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3145D
Unit Size: 1,000 Assay Points
Part Number: AL3145M
Unit Size: 10,000 Assay Points
Part Number: AL3145R
Unit Size: 50,000 Assay Points
  • Inositol Monophosphate (IP1) is part of the group of phosphorylated inositols that play a key role in diverse cellular functions such as cell growth, apoptosis, cell migration, endocytosis, and cell differentiation. Inositol 1,4,5 triphosphate (IP3) signals the activation of Gq coupled-receptors, however, it's short half-life makes it challenging to assess for drug screening. IP1 is a downstream metabolite of IP3, which can be made stable by the introduction of Lithium Chloride (LiCl). As such its accumulation following Gq-coupled receptor-induced phospholipase C (PLC) cascade allows for the direct characterization of all types of compounds acting on Gq-coupled receptors.

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Therapeutic Area
    Metabolic
    Unit Size
    1,000 Assay Points