Skip to main content

AlphaLISA Biotin-Free Human IL-6 Detection Kit, 5,000 Assay Points

The AlphaLISA Human IL-6 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-6 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3025F
Unit Size: 5,000 Assay Points
Part Number: AL3025HV
Unit Size: 100 Assay Points
Part Number: AL3025C
Unit Size: 500 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on the target cell analyzed and the in vivo environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to the gp130/ IL-6R receptor complex, leading to the activation of JAK/STAT, MAPK and PI3K cascades.

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Automation Compatible
    Yes
    Application
    High biotin samples
    Protein Analysis & Detection
    Assay Points
    5000
    Assay Target
    IL-6
    Assay Target Class
    Cytokine
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Therapeutic Area
    Inflammation
    Brand
    AlphaLISA
    Unit Size
    5,000 Assay Points

Resources

1-4 of 4 Resources
Application Note Icon Image
Application Note
Avoiding biotin interference in AlphaLISA assays
AlphaLISA, assays, biotin interferance
Technical Note
Biotin-Free AlphaLISA Assays for the Detection of Cytokines in PBMC Supernatants
RPMI, AlphaLISA, high biotin media, PBMCs, cytokines
Application Note Icon Image
Application Note
Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines
Cytokine, immune cells, cancer cell lines, biomarkers, checkpoint, application note
Flyer
Reagent solutions for autoimmunity research.
AlphaLISA, Alpha, HTRF, SureFire, autoimmunity, flyer