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AlphaLISA Human IL-1β Detection Kit, 500 Assay Points

AlphaLISA® Human Interleukin-1β (IL1β) Detection Kit is designed detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

We now offer a new and improved High Performance version of AlphaLISA Human IL1β Detection Kit, part number AL3160.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL220C
Unit Size: 500 Assay Points
Part Number: AL220F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Assay
    Cytokine
    Assay Points
    500
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Inflammation
    Unit Size
    500 Assay Points

Resources

1-3 of 3 Resources
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Application Note
Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

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Application Note
Tracking Inflammasome Activity via Measuring IL-1β levels with AlphaLISA and HTRF

It has been suggested that inflammation promotes malignancy via proinflammatory cytokines, such as IL-1ß, which enhance immune...