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AlphaLISA Biotin-Free Human TNFα Detection Kit, 500 Assay Points

The AlphaLISA® Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

We now offer a new and improved High Performance version of the AlphaLISA Biotin Free Human TNF.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL325HV
Unit Size: 100 Assay Points
Part Number: AL325C
Unit Size: 500 assay points
Part Number: AL325F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    Features and Benefits:

    • No-wash steps, no separation steps
    • Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Results in less than 3 hours

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    High biotin samples
    Protein Analysis & Detection
    Assay Points
    Assay Target Class
    Automation Compatible
    Detection Method
    Experimental Type
    In vitro
    Therapeutic Area
    Unit Size
    500 assay points


1-4 of 4 Resources
Application Note Icon Image
Application Note
Avoiding biotin interference in AlphaLISA assays

This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in...

Technical Note
Biotin-Free AlphaLISA Assays for the Detection of Cytokines in PBMC Supernatants

In this technical note, we will demonstrate the ease of quantitating secreted cytokines from biotin-enriched supernatants...

Application Note Icon Image
Application Note
Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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