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AlphaLISA High Performance Human IFNβ Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Human Interferon β (IFNβ) Detection Kit is designed for the quantitative determination of human IFNβ in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL3133.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3191HV
Unit Size:
Part Number: AL3191C
Unit Size:
Part Number: AL3191F
Unit Size:
  • Interferon-beta (IFNβ) is a cytokine produced in large quantities by fibroblasts in response to pathogens. IFNβ controls both immune responses to viral infections and nonspecific humoral immune responses. In a healthy environment, IFNβ is only present in small amounts. However, with viral infection, inflammation, and autoimmunity, levels can rise significantly. The biological effects of IFNβ include antiviral and antiproliferative properties. IFNβ has been utilized as a therapeutic agent in the treatment of multiple sclerosis and chronic active hepatitis B, with some success. If the disease has not been present for more than five years, it appears to have the best chance of success.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Inflammation