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AlphaLISA High Performance Human IFNγ Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Human IFNγ Detection Kit is designed for the quantitative determination of human IFNγ in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL217, the Human IFN-γ detection kit.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL3153HV
Unit Size: 100 Assay Points
List price: USD 896.90
Your price:
USD 896.90
USD 896.90 /each
Part number: AL3153C
Unit Size: 500 Assay Points
List price: USD 2,261.00
Your price:
USD 0.00
USD 2,261.00 /each
Part number: AL3153F
Unit Size: 5,000 Assay Points
List price: USD 14,900.00
Your price:
USD 0.00
USD 14,900.00 /each

Overview

Interferon gamma (IFNγ) is a homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells. It is critical for innate and adaptive immunity against viral, bacterial, and protozoan infections, and is involved in tumor growth. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Genetic variation of this cytokine is also associated with the risk of aplastic anemia.

Formats
  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features
  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay
  • Human IFN-gamma high sensitivity detection

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Analysis & Detection
Protein Detection
Assay Points
100
Assay Target Class
Cytokine
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Autoimmunity
Cardiovascular
Immuno-oncology
Inflammation
Oncology
Unit Size
100 Assay Points

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