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AlphaLISA High Performance Human IFNγ Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Human IFNγ Detection Kit is designed for the quantitative determination of human IFNγ in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL217, the Human IFN-γ detection kit.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3153HV
Unit Size: 100 Assay Points
Part Number: AL3153F
Unit Size: 5,000 Assay Points
Part Number: AL3153C
Unit Size: 500 Assay Points
  • Interferon gamma (IFNγ) is a homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells. It is critical for innate and adaptive immunity against viral, bacterial, and protozoan infections, and is involved in tumor growth. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Genetic variation of this cytokine is also associated with the risk of aplastic anemia.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay
    • Human IFN-gamma high sensitivity detection

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Automation Compatible
    Yes
    Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    100
    Assay Target
    IFN-γ
    Assay Target Class
    Cytokine
    Assay Technology
    Alpha
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Cardiovascular
    Immuno-oncology
    Inflammation
    Oncology
    Brand
    AlphaLISA
    Unit Size
    100 Assay Points

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