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AlphaLISA High Performance Biotin-Free Human IL-17A Detection Kit, 100 Assay Points

The AlphaLISA® High Performance (HP) Biotin-Free Human IL-17A Detection Kit is designed for the quantitative determination of human IL-17A in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL346.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3162HV
Unit Size: 100 Assay Points
Part Number: AL3162C
Unit Size: 500 Assay Points
Part Number: AL3162F
Unit Size: 5,000 Assay Points
  • Human Interleukin 17 (IL-17 or IL-17A) is a homodimer formed by two ~15 kDa subunits produced by a subset of T helper cells named Th17. It is a proinflammatory cytokine that enhances T-cell priming and stimulates macrophages, fibroblasts, endothelial, and epithelial cells to produce multiple mediators of inflammation such as IL-1, IL-6, TNF-α, NOS-2, metalloproteases, and chemokines. IL-17 has been implicated in the proinflammatory patterns associated with joint inflammation and rheumatoid arthritis (RA) in mouse and human models. It is also critical for neutrophil activation and migration, and induces IL-8, a key chemokine for neutrophils. IL-17 signals through IL-17R, which in mice has at least two members, IL-17RA, and IL-17RC. Recent studies suggest that the IL-17 pathway may be a novel therapeutic target for the treatment of chronic inflammatory diseases like asthma and RA.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Detection
    Assay Points
    100
    Assay Target Class
    Cytokine
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Inflammation
    Neuroscience
    Unit Size
    100 Assay Points

Resources

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Application Note
Homogeneous detection of cytokines from PBMC supernatants using AlphaLISA High Performance assays

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as...

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