The AlphaLISA® IgG1 Detection Kit is designed for the quantitative determination of human IgG1 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is a replacement for AL307.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 4.29 - 1,000,000 ng/mL |
Limit of Detection | 3.82 ng/mL |
Limit of Quantification | 4.29 ng/mL |
Sample Volume | 10 µL |
The AlphaLISA® IgG1 Detection Kit is designed for the quantitative determination of human IgG1 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is a replacement for AL307.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Immunoglobulin G1 (IgG1) belongs to the immunoglobulin antibody family and is the most abundant IgG subclass. IgG molecules are created and released by plasma B cells and represent an important mediated antibody response against viral pathogens by binding to soluble proteins and membrane protein antigens via variable domain and concomitantly activating effector mechanisms of the innate immune system. IgG1 can bind to C1q, causing complement-dependent cytotoxicity (CDC), and can bind to each of the different Fc receptors resulting in antibody-dependent cell-mediated cytotoxicity (ADCC). Increased IgG1 levels have been associated with inflammatory or autoimmune diseases that involve the central nervous system and IgG1 deficiencies are associated with a weakened immune system and increased susceptibility of infection.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
4.29 - 1,000,000 ng/mL
|
Limit of Detection |
3.82 ng/mL
|
Limit of Quantification |
4.29 ng/mL
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
IgG1
|
Target Class |
Biologics
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
100 Assay Points
|
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