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AlphaLISA Biotin-Free Human Factor VIII Detection Kit, 100 Assay Points

The AlphaLaISA® Human Factor VIII Biotin-Free Detection Kit is designed for the quantitative detection of human Factor VIII in buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL362HV
Unit Size: 100 Assay Points
Part Number: AL362C
Unit Size: 500 assay points
Part Number: AL362F
Unit Size: 5,000 Assay Points
  • Formats:

    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

    AlphaLISA features:

    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In this AlphaLISA assay, a DIG-labeled Anti-Analyte Antibody binds to the anti-DIG Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads,resulting in a sharp peak of light emission at 615 nm

    Factor VIII is a blood clotting protein which is also called anti-hemophilic factor (AHF). Factor VIII is produced in the liver and in the endothelial cells of other organs and tissues. It circulates in the bloodstream as an inactive form complexed with von Willebrand factor (vWF). In damaged blood vessels Factor VIII disassociates from vWF to become active Factor VIII. Activated Factor VIII initiates the cascade of chemical reactions to promote blood clotting at the site of vessel damage to prevent blood loss from the body. For this reason, it has been used as a blood clotting drug for patients with hemophilia to restore hemostasis. The present kit detects hFactor VIII in human serum, plasma, cell culture supernatants, and cell lysate, and tissue homogenates.

  • Application
    Protein Analysis & Detection
    Assay Points
    Assay Target Class
    Automation Compatible
    Detection Method
    Experimental Type
    In vitro
    Therapeutic Area
    Unit Size
    100 Assay Points


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Application Note
Avoiding biotin interference in AlphaLISA assays

This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in...