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AlphaLISA Bovine IgG2 Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG2 in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: AL532HV
Unit Size: 100 Assay Points
List price: USD 1,056.00
Your price:
USD 1,056.00
USD 1,056.00 /each
Part number: AL532C
Unit Size: 500 assay points
List price: USD 3,372.00
Your price:
USD 0.00
USD 3,372.00 /each
Part number: AL532F
Unit Size: 5,000 Assay Points
List price: USD 16,890.00
Your price:
USD 0.00
USD 16,890.00 /each



  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).


  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In bovine, the IgG class is further divided into two subclasses: IgG1 and IgG2. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgG1 is involved in response to a foreign antigen. The presence of IgG2 usually signifies a mature antibody response. IgG2 has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically, bovine serum and plasma samples contain about 5.0 to 13.5 mg/mL of IgG2. The present kit permits detection of bovine IgG2 (i.e. analyte) in bovine serum and plasma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


Protein Analysis & Detection
Assay Points
Assay Target Class
Automation Compatible
Detection Method
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Unit Size
100 Assay Points

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