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Immunoassay buffer, 10X, 10 mL

Pre-formulated buffer optimal for most AlphaLISA analyte detection assays. This buffer is used in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL000C
Unit Size:
Part Number: AL000F
Unit Size:
  • Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. AlphaLISA Immunoassay Buffer (IAB) is a broadly-applicable buffer, used in most AlphaLISA immunoassays. This buffer ontains detergent and low salt, so it can be used to lyse cells in some assays. It is generally sufficient to expose the protein epitopes of membrane proteins for Alpha assay detection.

    10X formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100 and 0.5% Proclin-300

  • Application
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Format
    Microplates

Resources

1-2 of 2 Resources
Technical Note
Developing a high throughput AlphaLISA assay for screening activity of biologics produced by engineered probiotic microbes
Biologics, Tenza, AlphaLISA, High Throughput Screening, protein therapeutics
Application Note Icon Image
Application Note
Screening for Inhibitors of PD-1 and PD-L1 Binding with AlphaLISA Technology
pd-1, pd-l1, binding, interaction, binding assay, pdl-1, pd1