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AlphaLISA Surefire Ultra Total VEGFR-2 Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra total VEGFR-2 assay. This control is already provided in the AlphaLISA SureFire Ultra total VEGF R2 assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TVGFR-A-L
Part Number: ALSU-TVGFR-A-L
  • This control lysate is intended for use with the AlphaLISA SureFire Ultra total VEGFR-2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HUVEC cells were grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.

  • Automation Compatible
    Yes
    Application
    Kinase
    Protein Analysis & Detection
    Assay Target
    VEGF2 Receptor
    Assay Target Class
    Protein
    Detection Method
    Alpha
    Brand
    AlphaLISA SureFire Ultra
    Unit Size
    Vial