Extra control lysate for the AlphaLISA SureFire Ultra total VEGFR-2 assay. This control is already provided in the AlphaLISA SureFire Ultra total VEGF R2 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA SureFire Ultra total VEGFR-2 assay. This control is already provided in the AlphaLISA SureFire Ultra total VEGF R2 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra total VEGFR-2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HUVEC cells were grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Total
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
VEGF2 Receptor
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
Are you looking for resources, click on the resource type to explore further.
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
We are here to answer your questions.