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AlphaLISA Surefire Ultra Human Phospho-VAV1 (Tyr174) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA® SureFire® Ultra Human Phospho-VAV1 (Tyr174) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-VAV1 (Tyr174) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PVAV1-A-L
Part Number: ALSU-PVAV1-A-L
  • This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-VAV1 (Tyr174) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Positive Control Lysate: Prepared from THP1 cells, seeded at 300 K/mL and grown for two days in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 2 x 106 cells/mL and treated with 2 mM pervanadate for 10 minutes. Cells were lysed with 5x SureFire Ultra Lysis Buffer.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Immuno-oncology
    Oncology
    Unit Size
    1 Vial

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...