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AlphaLISA SureFire Ultra Human Phospho-VAV1 (Tyr174) Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-VAV1 (Tyr174) assay is a sandwich immunoassay for quantitative detection of phospho-VAV1 (Tyr174) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PVAV1-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PVAV1-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PVAV1-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PVAV1-A-HV
Unit Size: 100 Assay Points
  • VAV1 is a Rho-family guanine nucleotide exchange factor that is encoded by a proto-oncogene, Vav Guanine Nucleotide Exchange factor 1. Wild-type VAV1 is recognized as a cardinal signal transducer protein in the hematopoietic system, where it is exclusively expressed. However, mutations in various domains of the VAV1 protein are present in human cancers, including T-cell leukemia/lymphoma, peripheral T-cell lymphomas, lung adenocarcinoma, and squamous cell carcinomas. VAV1 is also an activator of T and B cell receptor signaling, therefore making it a potential target for protein degradation therapies to combat autoimmune disease, such as multiple sclerosis.

    The AlphaLISA SureFire Human Phospho-VAV1 (Tyr174) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-VAV1 (Tyr174) in cellular lysates, using Alpha Technology.

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Assay Technology
    Automation Compatible
    AlphaLISA SureFire Ultra
    Detection Method
    Experimental Type
    In vitro
    Target Species
    Therapeutic Area
    Unit Size
    50,000 Assay Points


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