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AlphaLISA Surefire Ultra Human and Mouse Phospho-SMAD2 (Ser465/467), Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra phospho-SMAD2 assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PSM2-A-L
Part Number: ALSU-PSM2-A-L
  • This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 10 ng/mL rhTGFβ for 90min and lysed in 6 mL of 1X SureFire Ultra Lysis buffer.

  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    TGFß/SMAD
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Therapeutic Area
    Metabolic
    Unit Size
    Vial

Resources

1-1 of 1 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...