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AlphaLISA Surefire Ultra Human and Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ phospho-SMAD2 assay is a sandwich immunoassay for quantitative detection of SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PSM2-A500
Unit Size: 500 Assay Points
Part Number: ALSU-PSM2-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PSM2-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PSM2-A-HV
Unit Size: 100 Assay Points
  • Formats:

    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

    In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

    AlphaLISA SureFire Ultra™ kits are compatible with:

    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies

    Alpha SureFire kits can be used for:

    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    TGFß/SMAD
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Metabolic
    Unit Size
    10,000 Assay Points

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...