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AlphaLISA SureFire Ultra Human & Mouse Phospho-RSK1 (Ser380) Detection Kit, 500 Assay Points

The AlphaLISA™ SureFire® Ultra™ Phospho-RSK1 (Ser380) assay is a sandwich immunoassay for quantitative detection of Phospho-RSK1 (Ser380) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: ALSU-PRSK1-C500
Unit Size: 500 Assay Points
List price: USD 2,346.00
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USD 2,346.00
USD 2,346.00 /each
Part number: ALSU-PRSK1-C10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
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USD 0.00
USD 14,270.00 /each
Part number: ALSU-PRSK1-C50K
Unit Size: 50,000 Assay Points
List price: USD 46,060.00
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USD 0.00
USD 46,060.00 /each
Part number: ALSU-PRSK1-C-HV
Unit Size: 100 Assay Points
List price: USD 740.59
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USD 0.00
USD 740.59 /each

Overview

Ribosomal protein S6 kinases (RSK) are a family of broadly expressed serine/threonine kinases activated in response to mitogenic stimuli, including tumor-promoting growth factors and phorbol esters. RSKs are positioned downstream of the Raf-MEK-ERK signal transduction pathway, and are directly phosphorylated by ERK1 and ERK2extracellular kinases. Active RSKs appear to play a significant role in transcriptional regulation, translocation to the nucleus, and phosphorylation of c-Fos and CREBs.

Current models suggest that RSK activation requires a binding of active ERK1/2 to a docking site at the carboxyl end of RSK. ERK moored to RSK1 phosphorylate at several sites, including the CTD activation loop (carboxyl-terminal kinase domain). The CTD autophosphorylates Serine 380, a site in the central hydrophobic region.

The AlphaLISA™ SureFire® Ultra™ p-RSK1 (Ser380) assay is a sandwich immunoassay for the quantitative detection of phospho-RSK1 (phosphorylated on Ser380) in cellular lysates, using Alpha Technology.

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Assay Target
Rsk1
Assay Target Class
Phosphoproteins
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
500 Assay Points

Video gallery

Resources

1-4 of 4 Resources
Guide Icon
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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