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AlphaLISA Surefire Ultra Total RNA polymerase II Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Total RNA POL II assay is a sandwich immunoassay for quantitative detection of total RNA POL II in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-TRNAP-A500
Unit Size: 500 Assay Points
Part Number: ALSU-TRNAP-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-TRNAP-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-TRNAP-A-HV
Unit Size: 100 Assay Points
  • RNA Polymerase II, also known as RNAP II and Pol II, is one of three RNA polymerase enzymes found in the nucleus of eukaryotic cells. Pol II is responsible for unspooling the genetic program in the form of protein-coding mRNAs and some small non-coding RNAs. A wide range of transcription factors are required for Pol II to bind to upstream gene promoters and begin transcriptions. Inhibitors targeting Pol II transcription machinery are potential therapeutics in various cancers and complex diseases.

    The AlphaLISA SureFire Human and Mouse Total RNA POL II Detection Kit is a sandwich immunoassay for the quantitative detection of total RNA POL II in cellular lysates, using Alpha Technology.

    Formats
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Oncology
    Unit Size
    100 Assay Points

Resources

1-2 of 2 Resources
Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...