JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases which include JAK2, JAK3, and TYK2. Numerous cytokines and growth factors, such as IL-6, IL-4, IFN-α, GM-CSF, induce the phosphorylation of JAKs. Activated JAK subsequently phosphorylates additional targets, including both cytokine receptors and major substrates, STATs. The JAK/STAT signaling pathway stimulates cell proliferation, differentiation, migration, and apoptosis. Alterations to JAK/STAT signaling reduce cytokine induced pro-inflammatory responses, making it an attractive target for anti-inflammatory therapies.

The AlphaLISA SureFire Human Total JAK1 Detection Kit is a sandwich immunoassay for the quantitative detection of total JAK1 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening