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AlphaLISA SureFire Ultra Human and Mouse Phospho-IkBα (Ser32/36) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-IkBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IkBα (Ser32/36) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-PIKBA-A10K
Unit Size: 10,000 Assay Points
Part Number: ALSU-PIKBA-A-HV
Unit Size: 100 Assay Points
Part Number: ALSU-PIKBA-A50K
Unit Size: 50,000 Assay Points
Part Number: ALSU-PIKBA-A500
Unit Size: 500 Assay Points
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IkBα) is a member of a family of cellular proteins that function to inhibit NF-kB transcription factor. When IkBα is phosphorylated by IKKβ, this prevents IkBα from inhibiting NF-kB which then frees NF-kB and allows it to enter the nucleus of the cell, where it activates various genes involved in inflammation and other immune responses. The IKKβ signaling pathway is associated with numerous therapeutic areas, including diseases such as multiple sclerosis, rheumatoid arthritis, psoriasis, and many types of cancers.

    The AlphaLISA SureFire Ultra Human and Mouse Phospho-IkBα (Ser32/36) is a sandwich immunoassay for the quantitative detection of Phospho-IkBα (Ser32/36) in cellular lysates, using Alpha Technology.

    Formats:
    • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
    • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
    • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
    • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
    AlphaLISA SureFire Ultra kits are compatible with:
    • Cell and tissue lysates
    • Antibody modulators
    • Biotherapeutic antibodies
    Alpha SureFire kits can be used for:
    • Cellular kinase assays
    • Receptor activation studies
    • Screening
  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    10000
    Assay Target
    IkBα
    Assay Target Class
    Phospho-protein
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Mouse
    Therapeutic Area
    Autoimmunity
    Immuno-oncology
    Oncology
    Unit Size
    10,000 Assay Points

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
SureFire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx